Skip to main content

An official website of the United States government

4D-NUCLEOFECTOR SYSTEM AND THE 4D-NUCLEOFECTOR X UNITS

Waiver type - Procurement: Nonavailability

Agency: NATIONAL INSTITUTES OF HEALTH (7529) • Product Service Code (PSC) : 6640 | Last Modified: 01/21/2023

Procurement Summary

The 4D-Nucleofector Core Unit is the controlling unit of the 4D-Nucleofector System and the 4D-Nucleofector X Unit. The different functional units can be added in a modular way to the 4D-Nucleofector Core Unit, and each is suited for different applications. The 4D-Nucleofactor is the only transfection machine to deliver foreign DNA directly into nucleus of the cell. This system is known for the most efficient transfection system using non-viral transfection method for hard-to-transfect cells. For some of CRISPR/Cas project that requires insertion of rather large DNA, using 4D-Nucleofactor in the ES cell is unavoidable. The 4D-Nucleofector Core Unit is manufactured in Germany. The MGGM is requesting the purchase of a transfection nucleofector apparatus to aid in the existing CRISPR program. Currently, MGGM is incurring excessive costs by using two step method for inserting large DNA which requires huge number of mice for IVF, and cage space and time (up to 1 year). The 4D-Nucleofector X Unit is one of the four functional modules of the 4D-Nucleofector System. It supports Nucleofection of various cell numbers (2 x 104 to 2 x 107) cells in different formats. The 4D-Nucleofector X Unit is commercially available. The 4D-Nucleofector X Unit is also manufactured in Germany.

Waiver Rationale Summary

Based on the findings, it is determined that the 4D-Nucleofector System and the 4D-Nucleofector X Unit are not mined, produced, or manufactured in the United States in sufficient and reasonably available commercial quantities and of satisfactory quality. Accordingly, the requirement of the Buy American Act that acquisitions be made from domestic sources, and that it be of domestic origin, is not applicable to this acquisition, since the referenced acquisition is within the non-availability exception stated in the Buy American Act. The feasibility of foregoing the requirement or providing a United States manufactured substitute has been considered.

Did / Will the solicitation include one of the standard BAA provisions announcing the agency’s intention to provide a price preference for domestic end products and construction material?

Yes

Was a sources sought or Request for Information issued?

No

OMB Determination

Consistent with Policy

Product

LABORATORY EQUIPMENT AND SUPPLIES

NAICS

334516 - Analytical Laboratory Instrument Manufacturing

Date Submitted

01/05/2023

Procurement Instrument Identifier (PIID)

4D-NUCLEOFECTOR SYSTEM 4D-NUCLEOFECTOR X UNITS

Solicitation ID

RFQ-NIAID-2147815

Procurement Stage

Post-solicitation

Expected Maximum Duration of the Proposed Waiver

Instant Delivery Only

Waiver Coverage

Individual Waiver

Funding Agency

NATIONAL INSTITUTES OF HEALTH